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Testing the efficacy of topical antimicrobial treatments using a two-and five-species chronic wound biofilm model

journal contribution
posted on 22.11.2021, 14:48 by Andreea-Gabriela Nedelea, Rebecca L. Plant, Lori I. Robins, Sarah Maddocks

Aims

The effectiveness of commercially available wound dressings and a HOCl gel formulation was tested against two- and five-species biofilms in a dynamic in vitro chronic wound infection model.

Method

Two-species biofilms (Pseudomonas aeruginosa and Staphylococcus aureus) were cultured using a biofilm flow device and treated with wound dressings containing silver, iodine, polyhexamethylene biguanide, crystal violet or HOCl gel at 5 h. Five-species biofilms (P. aeruginosa, S. aureus, Enterococcus faecalis, Streptococcus pyogenes and Escherichia coli) were similarly cultured and treated with HOCl gel at 5 and 24 h. Multidose experiments used two- and five-species biofilms with HOCl applied at 24, 48 and 72 h.

Results

None of the treatments completely disrupted the biofilms and, with the exception of silver, bacteria recovered in number post-treatment. HOCl was most effective when applied to 24 h established biofilms with most activity against P. aeruginosa. Recovery post-treatment was negligible with HOCl applied at 24 h and multiple doses indicated that bacteria were not becoming tolerant to treatment.

Conclusions

Realistic models are necessary to test the effectiveness of antimicrobial wound treatments to ensure findings are clinically translatable. HOCl gel shows promise as a new topical antimicrobial for wounds, especially due to its ability to inhibit P. aeruginosa.

Significance and Impact of the Study

This study highlights a need for robust in vitro data to support development and use of wound treatments that can only be obtained from the refinement of realistic infection models. Furthermore, it indicates the potential use of HOCl gel for chronic wound management.

History

Published in

Journal of Applied Microbiology

Publisher

Wiley

Acceptance Date

26/07/2021

Publication Date

28/07/2021

Version

AM (Accepted Manuscript)

Citation

Nedelea, A.G., Plant, R.L., Robins, L.I. and Maddocks, S.E., (2021) 'Testing the efficacy of topical antimicrobial treatments using a two‐and five‐species chronic wound biofilm model', Journal of Applied Microbiology. doi: 10.1111/jam.15239

Print ISSN

1364-5072

Electronic ISSN

1365-2672

Cardiff Met Affiliation

Cardiff School of Sport and Health Sciences

Cardiff Met Authors

Sarah Maddocks

Cardiff Met Research Centre/Group

  • Microbiology & Infection

Copyright Holder

© The Publisher

Language

en

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