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TA-AgNPs/Alginate Hydrogel and Its potential application as a promising antibiofilm material against polymicrobial wound biofilms using a unique biofilm flow model

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posted on 2022-11-18, 10:00 authored by Oranee Srichaiyapol, Sarah Maddocks, Saengrawee Thammawithan, Sakda Daduang, Sompong Klaynongsruang, Rina Patramanon

 The presence of biofilm within a chronic wound may delay the healing process. Thus, control of biofilm formation and providing bactericidal effect are crucial factors for wound healing management. Alginate-based nanocomposite hydrogels have been suggested as dressing materials for wound treatment, which are employed as a biocompatible matrix. Therefore, in this study, we aimed to develop a biocompatible antimicrobial wound dressing containing AgNPs and demonstrate its efficacy against polymicrobial wound biofilms by using a biofilm flow device to simulate a chronic infected, exuding wound and specific wound environment. The results from agar well diffusion, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays showed that TA-AgNPs exhibited antibacterial activity against wound pathogens. Additionally, the Minimum Biofilm Eradication Concentration assay (MBEC) demonstrated it could impair biofilm formation. Importantly, our TA-AgNPs/Alginate hydrogel clearly showed antibacterial activities against Streptococcus pyogenes, Staphylococcus aureus and Pseudomonas aeruginosa. Furthermore, we used the biofilm flow device to test the topical antimicrobial hydrogel against a three-species biofilm. We found that TA-AgNPs/Alginate hydrogel significantly showed a 3–4 log reduction in bacterial numbers when applied with multiple doses at 24 h intervals, and was especially effective against the chronic wound pathogen P. aeruginosa. This work highlighted that the TA-AgNPs/Alginate hydrogel is a promising material for treating complex wound biofilms. 

History

Published in

Microorganisms

Publisher

MDPI

Version

  • VoR (Version of Record)

Citation

Srichaiyapol, Oranee, Sarah E. Maddocks, Saengrawee Thammawithan, Sakda Daduang, Sompong Klaynongsruang, and Rina Patramanon (2022) 'TA-AgNPs/Alginate Hydrogel and Its potential application as a promising antibiofilm material against polymicrobial wound biofilms using a unique biofilm flow model', Microorganisms 10 (11): 2279. https://doi.org/10.3390/microorganisms10112279

Electronic ISSN

2076-2607

Cardiff Met Authors

Sarah Maddocks

Cardiff Met Research Centre/Group

  • Microbiology & Infection

Copyright Holder

  • © The Authors

Language

  • en

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