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TA-AgNPs/Alginate Hydrogel and Its potential application as a promising antibiofilm material against polymicrobial wound biofilms using a unique biofilm flow model

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posted on 18.11.2022, 10:00 authored by Oranee Srichaiyapol, Sarah Maddocks, Saengrawee Thammawithan, Sakda Daduang, Sompong Klaynongsruang, Rina Patramanon

 The presence of biofilm within a chronic wound may delay the healing process. Thus, control of biofilm formation and providing bactericidal effect are crucial factors for wound healing management. Alginate-based nanocomposite hydrogels have been suggested as dressing materials for wound treatment, which are employed as a biocompatible matrix. Therefore, in this study, we aimed to develop a biocompatible antimicrobial wound dressing containing AgNPs and demonstrate its efficacy against polymicrobial wound biofilms by using a biofilm flow device to simulate a chronic infected, exuding wound and specific wound environment. The results from agar well diffusion, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays showed that TA-AgNPs exhibited antibacterial activity against wound pathogens. Additionally, the Minimum Biofilm Eradication Concentration assay (MBEC) demonstrated it could impair biofilm formation. Importantly, our TA-AgNPs/Alginate hydrogel clearly showed antibacterial activities against Streptococcus pyogenes, Staphylococcus aureus and Pseudomonas aeruginosa. Furthermore, we used the biofilm flow device to test the topical antimicrobial hydrogel against a three-species biofilm. We found that TA-AgNPs/Alginate hydrogel significantly showed a 3–4 log reduction in bacterial numbers when applied with multiple doses at 24 h intervals, and was especially effective against the chronic wound pathogen P. aeruginosa. This work highlighted that the TA-AgNPs/Alginate hydrogel is a promising material for treating complex wound biofilms. 

History

Published in

Microorganisms

Publisher

MDPI

Version

VoR (Version of Record)

Citation

Srichaiyapol, Oranee, Sarah E. Maddocks, Saengrawee Thammawithan, Sakda Daduang, Sompong Klaynongsruang, and Rina Patramanon (2022) 'TA-AgNPs/Alginate Hydrogel and Its potential application as a promising antibiofilm material against polymicrobial wound biofilms using a unique biofilm flow model', Microorganisms 10 (11): 2279. https://doi.org/10.3390/microorganisms10112279

Electronic ISSN

2076-2607

Cardiff Met Authors

Sarah Maddocks

Cardiff Met Research Centre/Group

  • Microbiology & Infection

Copyright Holder

© The Authors

Language

en

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