Nitrite-derived nitric oxide reduces hypoxia-inducible factor 1α-mediated extracellular vesicle production by endothelial cells
Extracellular vesicles (EVs) are small, spherical particles enclosed by a phospholipid bilayer (∼30–1000 nm) released from multiple cell types, and have been shown to have pathophysiological roles in a plethora of disease states. The transcription factor hypoxia-inducible factor-1 (HIF-1) allows for adaptation of cellular physiology in hypoxia and may permit the enhanced release of EVs under such conditions. Nitric oxide (NO) plays a pivotal role in vascular homeostasis, and can modulate the cellular response to hypoxia by preventing HIF-1 accumulation. We aimed to selectively target HIF-1 via sodium nitrite (NaNO2) addition, and examine the effect on endothelial EV, size, concentration and function, and delineate the role of HIF-1 in EV biogenesis.
Published inNitric Oxide
- AM (Accepted Manuscript)
CitationBurnley-Hall, N., Willis, G., Davis, J., Rees, D.A. and James, P.E. (2017) 'Nitrite-derived nitric oxide reduces hypoxia-inducible factor 1Î±-mediated extracellular vesicle production by endothelial cells', Nitric Oxide, 63, pp.1-12.
Cardiff Met Affiliation
- Cardiff School of Sport and Health Sciences
Cardiff Met AuthorsPhilip James
Cardiff Met Research Centre/Group
- Cardiovascular Metabolism and Inflammation
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