Cardiff Metropolitan University
Browse
32 - Hypoxia - accepted manuscript.pdf (362.9 kB)

Nitrite-derived nitric oxide reduces hypoxia-inducible factor 1α-mediated extracellular vesicle production by endothelial cells

Download (362.9 kB)
journal contribution
posted on 2022-12-19, 12:14 authored by Nicholas Burnley-Hall, Gareth Willis, Jessica Davis, D. Aled Rees, Philip James

 Extracellular vesicles (EVs) are small, spherical particles enclosed by a phospholipid bilayer (∼30–1000 nm) released from multiple cell types, and have been shown to have pathophysiological roles in a plethora of disease states. The transcription factor hypoxia-inducible factor-1 (HIF-1) allows for adaptation of cellular physiology in hypoxia and may permit the enhanced release of EVs under such conditions. Nitric oxide (NO) plays a pivotal role in vascular homeostasis, and can modulate the cellular response to hypoxia by preventing HIF-1 accumulation. We aimed to selectively target HIF-1 via sodium nitrite (NaNO2) addition, and examine the effect on endothelial EV, size, concentration and function, and delineate the role of HIF-1 in EV biogenesis. 

History

Published in

Nitric Oxide

Publisher

Elsevier

Version

  • AM (Accepted Manuscript)

Citation

Burnley-Hall, N., Willis, G., Davis, J., Rees, D.A. and James, P.E. (2017) 'Nitrite-derived nitric oxide reduces hypoxia-inducible factor 1α-mediated extracellular vesicle production by endothelial cells', Nitric Oxide, 63, pp.1-12.

Print ISSN

1089-8603

Cardiff Met Affiliation

  • Cardiff School of Sport and Health Sciences

Cardiff Met Authors

Philip James

Cardiff Met Research Centre/Group

  • Cardiovascular Metabolism and Inflammation

Copyright Holder

  • © The Publisher

Language

  • en

Usage metrics

    Cardiovascular Health & Ageing - Journal Articles

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC