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Moderate‑intensity exercise alters markers of alternative activation in circulating monocytes in females: a putative role for PPARγ

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posted on 2022-12-12, 12:49 authored by J. S. Ruffino, N. A. Davies, Keith Morris, M. Ludgate, L. Zhang, Richard Webb, A. W. Thomas

 

Purpose: Monocytes may be primed towards differentiation into classically activated M1 macrophages or alternatively activated M2 macrophages. M1 macrophages greatly contribute to the inflammation which promotes insulin resistance, whereas M2 macrophages resolve inflammation. We have previously shown that exercise increases M2 marker expression in mixed mononuclear cells, possibly via activation of the nuclear transcription factor PPARγ. However, these effects have not been demonstrated specifically within monocytes. Thus, we aimed to investigate whether moderate-intensity exercise elicited similar effects on monocytic M1/M2 marker expression and PPARγ activity to those reported previously in mononuclear cells, so as to further elucidate the mechanisms by which exercise may alter inflammatory status and, accordingly, prevent insulin resistance.

Methods/results: 19 sedentary females completed an 8 week moderate-intensity exercise programme (walking 45 min, thrice weekly). Monocytes were isolated from blood via immunomagnetic separation; monocyte expression of M2 markers (Dectin-1: 2.6 ± 1.9-fold; IL-10: 3.0 ± 2.8-fold) significantly increased, whilst the expression of the M1 marker MCP-1 significantly decreased (0.83 ± 0.2 cf. basal), over the duration of the programme. Serum PPARγ activity levels and PPARγ target-genes (CD36: 1.9 ± 1.5-fold; LXRα: 5.0 ± 4.7-fold) were significantly increased after the 8 week exercise programme. Associated with these effects were significant improvements in systemic insulin sensitivity (McAuley's ISI: Δ0.98 M/mU/L cf. basal).

Conclusion: Exercise participation suppressed M1 markers and induced M2 markers in monocytes, potentially via PPARγ-triggered signalling, and these effects may contribute (perhaps via priming of monocytes for differentiation into M2 tissue-macrophages) to improved systemic insulin sensitivity in exercising participants. These findings provide an alternative mechanism by which exercise may exert its anti-inflammatory effects in order to prevent insulin resistance and type 2 diabetes.

History

Published in

European Journal of Applied Physiology

Publisher

Springer

Version

  • VoR (Version of Record)

Citation

Ruffino, J.S., Davies, N.A., Morris, K., Ludgate, M., Zhang, L., Webb, R. and Thomas, A.W. (2016) 'Moderate-intensity exercise alters markers of alternative activation in circulating monocytes in females: a putative role for PPARγ', European Journal of Applied Physiology, pp.1-12.

Print ISSN

1439-6319

Electronic ISSN

1439-6327

Cardiff Met Affiliation

  • Cardiff School of Sport and Health Sciences

Cardiff Met Research Centre/Group

  • Cellular Senescence and Pathophysiology

Copyright Holder

  • © The Authors

Language

  • en

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