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Download fileIncreased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles
journal contribution
posted on 2022-03-29, 13:23 authored by Rachel A. Adams, Ameena Al-Mosawi, Kelly Bérubé, Tim Jones, Timothy Higgins, Shelley-Ann EvansBACKGROUND: Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood. OBJECTIVE: The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leukocyte flow properties by measurement of polymorphonucleocyte (PMN) and monocyte actin polymerisation. METHODS: Rats were exposed to particulate air pollution by intratracheal instillation of PM10. Blood was collected from test and control animals at 3 days (n=10) and 6 weeks (n=10) after dust instillation. Partial differential leukocyte counts were performed. The intracellular F-actin content of blood PMNs and monocytes was determined by staining with FITC-phalloidin and flow cytometric determination of mean florescence intensity (MFI). RESULTS: There were no significant changes in PMN MFI (p=0.369, ANOVA) or cell counts (p=0.753, ANOVA). There was a significant increase in monocyte MFI (p=0.004, ANOVA) and a decrease in monocyte cell count (p=0.003, ANOVA) in instilled rats. CONCLUSIONS: Intratracheal instillation of air pollution particles resulted in an increase in blood monocyte actin polymerisation, which may cause trapping of monocytes. This could be a mechanism by which exposure to air pollution increases the risk of cardiovascular disease.
History
Published in
BiorheologyPublisher
IOS PressVersion
- VoR (Version of Record)
Citation
Adams, R.A., Al-Mosawi, A., Berube, K., Jones, T., Higgins, T. and Evans, S-A (2014) 'Increased monocyte actin polymerization in rat blood after intratracheal instillation of air pollution particles', Biorheology, 51, pp. 329-338Print ISSN
0006-355XCardiff Met Affiliation
- Cardiff School of Sport and Health Sciences
Cardiff Met Authors
Rachel A. Adams Shelley-Ann EvansCardiff Met Research Centre/Group
- Cardiovascular Metabolism and Inflammation
Copyright Holder
- © The Authors
Language
- en