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Foetal Ureaplasma parvum bacteraemia as a function of gestation-dependent complement insufficiency: Evidence from a sheep model of pregnancy

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posted on 2022-12-12, 16:37 authored by Matthew W. Kemp, Shatha Ahmed, Mike BeetonMike Beeton, Matthew S. Payne, Masatoshi Saitou, Yuichiro Miura, Haruo Usuda, Suhas G. Kallapur, Boris W. Kramer, Sarah J. Stock, Alan H. Jobe, John P. Newnham, Owen B. Spiller

 

Problem

Complement is a central defence against sepsis, and increasing complement insufficiency in neonates of greater prematurity may predispose to increased sepsis. Ureaplasma spp. are the most frequently cultured bacteria from preterm blood samples.

Method of study

A sheep model of intrauterine Ureaplasma parvum infection was used to examine in vivo Ureaplasma bacteraemia at early and late gestational ages. Complement function and Ureaplasma killing assays were used to determine the correlation between complement potency and bactericidal activity of sera ex vivo.

Results

Ureaplasma was cultured from 50% of 95-day gestation lamb cord blood samples compared to 10% of 125-day gestation lambs. Bactericidal activity increased with increased gestational age, and a direct correlation between functional complement activity and bactericidal activity (R2=.86; P<.001) was found for 95-day gestational lambs.

Conclusions

Ureaplasma bacteraemia in vivo was confined to early preterm lambs with low complement function, but Ureaplasma infection itself did not diminish complement levels.

History

Published in

American Journal of Reproductive Immunology

Publisher

Wiley

Version

  • AM (Accepted Manuscript)

Citation

Kemp, M.W., Ahmed, S., Beeton, M.L., Payne, M.S., Saito, M., Miura, Y., Usuda, H., Kallapur, S.G., Kramer, B.W., Stock, S.J. and Jobe, A.H. (2017) 'Foetal Ureaplasma parvum bacteraemia as a function of gestation‐dependent complement insufficiency: Evidence from a sheep model of pregnancy', American Journal of Reproductive Immunology, 77(1), p.e12599.

Print ISSN

1046-7408

Electronic ISSN

1600-0897

Cardiff Met Affiliation

  • Cardiff School of Sport and Health Sciences

Cardiff Met Authors

Michael Beeton Mike Beeton

Cardiff Met Research Centre/Group

  • Microbiology & Infection

Copyright Holder

  • © The Publisher

Language

  • en

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