Cuthbertson 2014 Ectopic lipid storage in Non-Alcoholic Fatty Liver Disease is not mediated by impaired mitochondrial oxidative capacity in skeletal muscle.pdf (320.28 kB)
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Ectopic lipid storage in non-alcoholic fatty liver disease is not mediated by impaired mitochondrial oxidative capacity in skeletal muscle

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posted on 16.05.2022, 16:59 authored by DJ Cuthbertson, A Irwin, VS Sprung, H Jones, Christopher J. A. Pugh, C Daousi, VL Adams, WE Bimson, F Shojaee-Moradie, AM Umpleby, JP Wilding, GJ Kemp

 Non-alcoholic fatty liver disease (NAFLD), characterized by lipid deposition within the liver [intrahepatocellular lipid (IHCL)], is associated with insulin resistance and the metabolic syndrome (MS). It has been suggested that impaired skeletal muscle mitochondrial function may contribute to ectopic lipid deposition, and the associated MS, by altering post-prandial energy storage. To test this hypothesis, we performed a cross-sectional study of 17 patients with NAFLD [mean±S.D.; age, 45±11 years; body mass index (BMI), 31.6±3.4 kg/m2] and 18 age- and BMI-matched healthy controls (age, 44±11 years; BMI, 30.5±5.2 kg/m2). We determined body composition by MRI, IHCL and intramyocellular (soleus and tibialis anterior) lipids (IMCLs) by proton magnetic resonance spectroscopy (1H-MRS) and skeletal muscle mitochondrial function by dynamic phosphorus magnetic resonance spectroscopy (31P-MRS) of quadriceps muscle. Although matched for BMI and total adiposity, after statistical adjustment for gender, patients with NAFLD (defined by IHCL ≥ 5.5%) had higher IHCLs (25±16% compared with 2±2%; P<0.0005) and a higher prevalence of the MS (76% compared with 28%) compared with healthy controls. Despite this, the visceral fat/subcutaneous fat ratio, IMCLs and muscle mitochondrial function were similar between the NAFLD and control groups, with no significant difference in the rate constants of post-exercise phosphocreatine (PCr) recovery (1.55±0.4 compared with 1.51±0.4 min−1), a measure of muscle mitochondrial function. In conclusion, impaired muscle mitochondrial function does not seem to underlie ectopic lipid deposition, or the accompanying features of the MS, in patients with NAFLD 

History

Published in

Clinical Science

Publisher

Portland Press

Version

AM (Accepted Manuscript)

Citation

Cuthbertson, D.J., Irwin, A., Sprung, V.S., Jones, H., Pugh, C.J., Daousi, C., Adams, V.L., Bimson, W.E., Shojaee-Moradie, F., Richardson, P.,Umpleby, A.M., Wilding, J. & Kemp, G. (2014) 'Ectopic lipid storage in Non-Alcoholic Fatty Liver Disease is not mediated by impaired mitochondrial oxidative capacity in skeletal muscle', Clinical Science, 127(12), pp.655-663

Print ISSN

0143-5221

Electronic ISSN

1470-8736

Cardiff Met Affiliation

  • Cardiff School of Sport and Health Sciences

Cardiff Met Authors

Christopher Pugh

Cardiff Met Research Centre/Group

  • Cardiovascular Physiology

Copyright Holder

© The Publisher

Language

en