Binding of two bacterial biofilms to dialkyl carbamoyl chloride (DACC)-coated dressings in vitro

<p>Objective:</p> <p>To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing <em>in vitro</em>.</p> <h2>Method:</h2> <p>Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either <em>Pseudomonas aeruginosa</em> or methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm.</p> <h2>Results:</h2> <p><em>Pseudomonas aeruginosa</em> biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples.</p> <h2>Conclusion:</h2> <p>Biofilms of two different test bacteria bound to dressings <em>in vitro</em> with the DACC-coating on the dressings enhancing the binding of MRSA biofilm.</p> <h2>Declaration of interest:</h2> <p>This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.</p>