A new structural biomarker that quantifies and predicts changes in clot strength -Morris R.pdf (545.26 kB)
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A new structural biomarker that quantifies and predicts changes in clot strength and quality in a model of progressive haemodilution

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journal contribution
posted on 25.03.2022, 16:07 authored by Matthew J. Lawrence, Sendhil Kumar, Karl Hawkins, Stuart Boden, Harvey Rutt, Gavin Mills, Ahmed Sabra, Keith Morris, Simon J. Davidson, Nafieseh Badiei, Martin R. Brown, Phylip R. Williams, Phillip A. Evans
We investigated the effect of progressive haemodilution on the dynamics of fibrin clot formation and clot microstructure using a novel rheological method. The technique measures clotting time (TGP), clot strength (G`GP), and quantifies clot microstructure (df) at the incipient stages of fibrin formation. We use computational modelling to examine the relationship between structure and mass, as well as helium ion microscopy (HIM) to compare morphological changes in the fully formed clot to that of the incipient clot. Methods This is an in vitro study; 90 healthy volunteers were recruited with informed consent and a 20 ml sample of whole blood obtained from each volunteer. Five clinically relevant dilutions were investigated using 0.9w.v isotonic saline (0, 10, 20, 40 and 60%, n = 18 for each dilution). The rheological method of assessing structural clot changes was compared against conventional coagulation screen and fibrinogen estimation. Results Fractal dimension (df) and final clot microstructure both decreased with progressive dilution (significant at a dilution of 20%) with similar relationships observed for final clot characteristics in HIM images. Significant correlations were observed between df and G`GP (clot strength) (0.345, p = 0.02), as well as clotting time (PT: − 0.690, p > 0.001; APTT: − 0.672, p > 0.001; TGP: − 0.385, p = 0.006). Conclusions This study provides new insight into the effects of haemodilution by isotonic saline on clotting time (TGP), clot strength (G′GP) and clot microstructure (df). Previous studies have attempted to link clot microstructure to clot quality/strength, however this study provides a significant step in quantifying these relationships. Abbreviations df, fractal dimension; GP, gel point; TGP, time to gel point; G`GP, clot strength

History

Published in

Thrombosis research

Publisher

Elsevier

Version

AM (Accepted Manuscript)

Citation

Lawrence, M.J., Kumar, S., Hawkins, K., Boden, S., Rutt, H., Mills, G., Sabra, A., Morris, R.H.K., Davidson, S.J., Badiei, N., Brown, M.R., Williams, P.R. & Evans, P.A. (2014) 'A new structural biomarker that quantifies and predicts changes in clot strength and quality in a model of progressive haemodilution', Thrombosis research, 134(2), pp.488-494

Print ISSN

0049-3848

Electronic ISSN

1879-2472

Cardiff Met Affiliation

  • Cardiff School of Sport and Health Sciences

Cardiff Met Authors

Keith Morris

Cardiff Met Research Centre/Group

  • Cardiovascular Metabolism and Inflammation

Copyright Holder

© The Publisher

Language

en