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A genome-led study on the pathogenesis of Fusobacterium necrophorum infections

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posted on 2022-08-09, 10:06 authored by Gary Thapa, Ambikesh Jayal, Elvis Sikazwe, Thomas Perry, Ali Mohammed Al Balushi, Paul Livingstone

 Fusobacterium necrophorum causes a range of mild to life threatening infections and there is uncertainty in terms of diagnosis and treatment due to the lack of knowledge on their pathogenic mechanisms. This study charac-terised genomes of F. necrophorum to compare their virulence factors and investigate potential infection markers. 27 isolates of F. necrophorum from patients with pharyngotonsillitis were subjected to whole genome sequencing and compared with 42 genomes published in the NCBI database. Phylogenomics, pangemome, pan-GWAS and virulome were analysed to study strain variations with reference to virulence factors. Core genome based phy-logenomic tree exhibited three clades of which Clade A belonged to F. necrophorum subsp necrophorum, clades B and C were F. necrophorum subsp funduliforme. Pan-GWAS and Pan-Virulome suggest some marker genes asso-ciated with clinical sources of isolation that needs further validation. Our study highlights some interesting features of the pathogenesis of F. necrophorum infections. Although the animal isolate genomes had some marker genes, the genomes of human isolates did not exhibit clear correlation to their clinical sources of isolation. This prompts to  think of  other mechanisms such as  co-infections or  host factors that can be  involved in  the pathogenesis.    

History

Published in

Gene

Publisher

Elsevier

Version

  • VoR (Version of Record)

Citation

Thapa, G., Jayal, A., Sikazwe, E., Perry, T., Al Balushi, A.M. and Livingstone, P. (2022) 'A genome-led study on the pathogenesis of Fusobacterium necrophorum infections', Gene, p.146770.

Print ISSN

0378-1119

Cardiff Met Affiliation

  • Cardiff School of Sport and Health Sciences

Cardiff Met Authors

Paul Livingstone

Cardiff Met Research Centre/Group

  • Microbiology & Infection

Copyright Holder

  • © The Authors

Language

  • en

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